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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
04/10/2011 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SALLES, H. O.; VASCONCELOS, I. M.; SANTOS, L. F. L.; OLIVEIRA, H. D.; COSTA, P. P. C.; NASCIMENTO, N. R. F.; SANTOS, C. F.; SOUSA, D. F.; JORGE, A. R. C.; MENEZES, D. B.; MONTEIRO, H. S. A.; GONDIM, D. M. F.; OLIVEIRA, J. T. A. |
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Afiliação: |
HEVILA OLIVEIRA SALLES FIGUEIREDO, CNPC; Universidade Federal do Ceará (UFC), Fortaleza, CE.; Universidade Estadual do Ceará (UECe) - Fortaleza, CE.; UECe; UECe; UECe; UECe; UFC, Fortaleza, CE.; UFC; UFC; UFC; UFC; UFC. |
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Título: |
Towards a better understanding of Ipomoea asarifolia toxicity: evidence of the involvement of a leaf lectin. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Toxicon, v. 58, p. 502-508, 2011. |
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DOI: |
10.1016/j.toxicon.2011.08.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination of the kidneys perfused with LEF exhibited little alterations. These toxic effects observed above were concomitant with the increase of LEF hemag-glutination activity, which strongly suggest that one of the toxic principles of I. asarifolia is a lectin present in its leaves. MenosAbstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination o... Mostrar Tudo |
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Palavras-Chave: |
Ipomoea asarifolia; Lectin; Sulato de amônia. |
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Thesagro: |
Erva daninha; Lectina; Planta tóxica; Salsa; Toxidez. |
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Thesaurus Nal: |
toxicity. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02855naa a2200385 a 4500
001 1902176
005 2019-09-23
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1016/j.toxicon.2011.08.011$2DOI
100 1 $aSALLES, H. O.
245 $aTowards a better understanding of Ipomoea asarifolia toxicity$bevidence of the involvement of a leaf lectin.$h[electronic resource]
260 $c2011
520 $aAbstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination of the kidneys perfused with LEF exhibited little alterations. These toxic effects observed above were concomitant with the increase of LEF hemag-glutination activity, which strongly suggest that one of the toxic principles of I. asarifolia is a lectin present in its leaves.
650 $atoxicity
650 $aErva daninha
650 $aLectina
650 $aPlanta tóxica
650 $aSalsa
650 $aToxidez
653 $aIpomoea asarifolia
653 $aLectin
653 $aSulato de amônia
700 1 $aVASCONCELOS, I. M.
700 1 $aSANTOS, L. F. L.
700 1 $aOLIVEIRA, H. D.
700 1 $aCOSTA, P. P. C.
700 1 $aNASCIMENTO, N. R. F.
700 1 $aSANTOS, C. F.
700 1 $aSOUSA, D. F.
700 1 $aJORGE, A. R. C.
700 1 $aMENEZES, D. B.
700 1 $aMONTEIRO, H. S. A.
700 1 $aGONDIM, D. M. F.
700 1 $aOLIVEIRA, J. T. A.
773 $tToxicon$gv. 58, p. 502-508, 2011.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
23/11/2015 |
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Data da última atualização: |
22/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LOPES, S. da S.; LANA, U. G. de P.; ALVES, M. de C.; PALHARES, P. L. S.; MAGALHAES, J. V.; GUIMARAES, C. T.; CARNEIRO, A. A.; SOUSA, S. M. de. |
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Afiliação: |
UBIRACI GOMES DE PAULA LANA, CNPMS; MEIRE DE CASSIA ALVES, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; ANDREA ALMEIDA CARNEIRO, CNPMS; SYLVIA MORAIS DE SOUSA TINOCO, CNPMS. |
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Título: |
Tobacco transformation with rice Phosphorus-Starvation Tolerance 1 gene and its sorghum and maize homologs. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstracts. [S.l.]: International Society for Plant Molecular Biology, 2015. p. 166. |
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Idioma: |
Inglês |
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Thesagro: |
Fósforo; Genética; Milho; Sorgo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/133713/1/Tobacco-transforrmation.pdf
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Marc: |
LEADER 00814nam a2200229 a 4500
001 2029274
005 2016-02-22
008 2015 bl uuuu u00u1 u #d
100 1 $aLOPES, S. da S.
245 $aTobacco transformation with rice Phosphorus-Starvation Tolerance 1 gene and its sorghum and maize homologs.$h[electronic resource]
260 $aIn: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstracts. [S.l.]: International Society for Plant Molecular Biology, 2015. p. 166.$c2015
650 $aFósforo
650 $aGenética
650 $aMilho
650 $aSorgo
700 1 $aLANA, U. G. de P.
700 1 $aALVES, M. de C.
700 1 $aPALHARES, P. L. S.
700 1 $aMAGALHAES, J. V.
700 1 $aGUIMARAES, C. T.
700 1 $aCARNEIRO, A. A.
700 1 $aSOUSA, S. M. de
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Embrapa Milho e Sorgo (CNPMS) |
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